Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared
to other thermostable polymerase. Its molecular weight is 90 kDa.
It can amplify DNA target up to 2 kb（simple template）. The elongation velocity is 0.2~0.4kb/min(70~75°C). Pfu DNA
polymerase possesses 3' to 5' exonuclease proofreading activity
that enables the polymerase to correct nucleotide-misincorporation
errors. This means that Pfu DNA polymerase-generated PCR fragments
will have fewer errors than Taq-generated PCR inserts. Using Pfu
DNA polymerase in your PCR reactions results in blunt-ended PCR
products, which are ideal for cloning into blunt-ended vectors. Pfu
DNA polymerase is superior for techniques that require
high-fidelity DNA synthesis.